2020/12/21 12:03:21
多樣品組織研磨儀研磨破碎黃芪實(shí)驗(yàn)-上海凈信
Source：Shanghai Jingxin
本研究從富含多糖和次生物質(zhì)的黃芪干燥根提取高質(zhì)量的基因組DNA,為今后黃芪遺傳多樣性、分子鑒定研究奠定基礎(chǔ)。
實(shí)驗(yàn)步驟
需了解具體步驟，請致電廠家
實(shí)驗(yàn)結(jié)果
采用改良的CTAB法、高鹽低pH法、SDS法、苯酚法等多種提取液提取黃芪干燥根的基因組 DNA,結(jié)果表明,CTAB法和高鹽低pH法能得到高質(zhì)量的基因組DNA。用這兩種方法提取多批黃芪干燥根,瓊脂糖凝膠電泳條帶清晰,降解較少,能有效的從黃芪干藥材中提取到高質(zhì)量的基因組DNA。將DNA以引物5S進(jìn)行PCR擴(kuò)增,獲得清晰的條帶。
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